Which trials can I do, and how do I set them up?
Last reviewed 16 October 2026. Source: EN 17700-1 to EN 17700-5:2024 (Plant biostimulants — Claims); Regulation (EU) 2019/1009, Annex I PFC 6 and Annex IV. Selected facts summarised in original wording; consult the official standards for the full conditions.
In simple words
Trials can take place under farming conditions or in a more controlled environment. Field trials show performance in practice. Controlled trials can help investigate a particular effect. The right choice depends on your claim and the evidence needed for assessment; not every trial type suits every claim.
Field trials
Field trials investigate the product under farming conditions. They help show how it performs amid differences in weather, soil and crop growth.
The design needs to account for that variation so the result does not simply reflect a better part of the field.
Trial approaches at a glance
| Approach | What it helps investigate | Main limitation |
|---|---|---|
| Field trial | Performance under farming conditions, including natural weather and soil variation. | Variation needs a suitable layout, repeated comparisons and analysis. |
| Strip trial | Product responses over larger practical farm areas. | Differences between strips can obscure the product effect. |
| Controlled-condition trial | A focused effect under more closely managed conditions. | A pot or chamber result may not represent farm performance. |
| Coordinated trial programme | Consistency across relevant sites, seasons or conditions. | Results need compatible questions and methods to be interpreted together. |
A trial programme is a way of organising several studies, not a separate growing environment. Suitability for assessment depends on the claim and chosen approach.
The building blocks of a trial
| Design choice | Why it matters |
|---|---|
| A suitable control | Gives a fair basis for comparison, including nutrient effects where relevant. |
| Random allocation and blocks | Help prevent position or soil gradients favouring one treatment. |
| Repeated treatment plots | Help estimate natural variation within a trial. |
| Suitable plot size and measured area | Reduce edge effects and allow realistic application and harvest. |
| Dose and timing | Link the trial to the proposed product use. |
| Planned measurements and analysis | Keep the study focused on the claim rather than the most favourable result. |
The precise layout, number of repeats and dimensions depend on the crop, claim and applicable provisions.
Strip trials
Strip trials compare larger areas under farming conditions. They can be practical for commercial farms, but differences across the field can make results harder to interpret.
Ask how they fit into the wider evidence programme before relying on them for assessment.
Controlled-condition trials
Growth rooms, climate chambers and other controlled environments allow researchers to study an effect more closely.
They can help answer a focused question, but a result in controlled conditions does not automatically show performance on a farm. Agree whether this approach is suitable for your claim.
A programme rather than an isolated result
Trials across different places or seasons can give a broader picture. Combining results needs careful planning; separate studies cannot always be treated as one programme.
What a good design should achieve
The comparison should make it possible to identify the product’s effect. The use tested should fit the intended label, and the measurements should answer the claim.
A qualified trial specialist should plan how to handle natural variation and analyse the results. The detailed design belongs in the trial plan, not in this overview.
Keep the work understandable
Keep the records behind the report and explain important changes or problems. The assessor needs to understand what was tested and how the conclusions were reached.
A fair comparison in practice
For example, a wheat trial might compare the product with a treatment that does not receive it, while keeping the variety and normal crop management comparable. If the product supplies nutrients, the comparison also needs to address that difference.
Putting all treated plots at the fertile end of a field would make the result difficult to interpret. Random allocation helps avoid favouring a treatment. Blocks can help organise comparisons across a soil gradient.
These are general experimental design ideas, not a complete protocol. The specialist must choose a layout appropriate to the site and claim.
Why repeats and plot size matter
Repeated treatment plots give a picture of natural variation. Without that picture, a small difference between treatments can look more convincing than it is.
The area measured must also be suitable for the crop and practical operations. Edge effects, uneven application and the way the crop is harvested can all influence results. Bigger plots do not automatically make a weak design reliable.
The official standards contain precise design provisions. Ask the provider to explain how its proposed design meets the chosen approach rather than copying a generic layout.
Some numerical starting points from EN 17700
These selected figures help you understand a provider’s proposal. They summarise EN 17700-1:2024 in original wording; they are not a complete design.
| What you are comparing | Number to discuss |
|---|---|
| Repeated treatments in a field trial | At least 4 replicates per treatment in the same field. |
| Controlled-condition and protected-crop tests | At least 3 plants per replicate. Plants and replicates are different counts. |
| A field-trial series | At least 4 independent trials, or at least 15 strip trials, for series analysis. |
| Strip trials used in a series | The standard does not require within-trial replicates for this specific approach. |
For example, two treatments with four repeated plots each give eight plots, but still only one trial. The total trial programme must separately cover the intended claim and crops.
Controlled-condition trials are not part of the field-trial-series route. Also distinguish a greenhouse used under normal crop-growing practice from an experiment that deliberately controls the environment.
Choose the main measurement before starting
Decide which result will answer the claim, and when it will be measured. For a quality question, harvest weight alone may miss the trait you wanted to show.
Plan the analysis before seeing the results. Record the size of the effect and the variation, not only whether a statistical test passes. A statistically convincing result can still have little practical value, and a promising difference may remain too uncertain.
What do the statistical numbers mean?
EN 17700-1:2024 sets a significance threshold of p < 0.15 for individual field or protected-crop trials and for field-trial-series analysis. For individual trials under controlled conditions, it uses p < 0.10.
These correspond to the standard’s stated minimum confidence levels of 85% and 90%. They are not an 85% or 90% probability that the product works. A p-value concerns how compatible the observed result is with the statistical no-effect model, given the assumptions.
Ask the specialist to explain both the effect size and the uncertainty. Passing a threshold does not replace a fair design, meaningful measurements or a useful agronomic effect.
From trial plan to report
The plan should link the product, intended use and claim to the comparison and measurements. During the trial, keep enough information to explain the conditions and any departures from the plan.
The report should make it possible to follow the evidence from the original measurements to the conclusion. Photos add context, but do not replace comparable measurements.
Common setup mistakes
- Comparing fields with different management as if the product were the only difference.
- Changing the main measurement after seeing which result looks best.
- Drawing a farm-performance conclusion from a small exploratory pot test.
- Keeping a graph but losing the data behind it.
Choosing a trial provider
The company doing the trials should be able to show that its staff are competent and that the work follows suitable quality criteria. Check this before commissioning the work.
Certification is not a blanket requirement for every biostimulant efficacy trial. Ask your notified body whether it requires a particular certification, accreditation or recognised quality scheme for your assessment. A certificate alone does not guarantee acceptance.
Where do the standards fit?
The EN 17700 series provides a recognised framework for evidence supporting plant biostimulant claims. Using standards is voluntary; the product must still meet the legal requirements.
This article combines original explanations with selected numerical and scope requirements from the standards. It is not a complete protocol or a substitute for the official standards and their full conditions. Agree the chosen approach with a qualified trial specialist and your notified body.
Common questions
Are greenhouse and field trials interchangeable?
Not automatically. The growing conditions and the question being tested matter. Discuss which approach fits your intended claim.
Can I submit a strip trial?
Ask your notified body how it can contribute to the evidence. A useful farm comparison is not automatically sufficient for assessment.
Words used in this article (9)
- Plant biostimulant (PFC 6)
- A product that helps plants take up and use nutrients, whatever amount of nutrients the product itself contains.
- PFC (product function category)
- A group that says what the product does. There are 7 groups: fertiliser (PFC 1), liming material (PFC 2), soil improver (PFC 3), growing medium (PFC 4), inhibitor (PFC 5), plant biostimulant (PFC 6) and fertilising product blend (PFC 7).
- FPR (Fertilising Products Regulation)
- The EU law that sets the rules for selling fertilising products with a CE marking in all EU countries. Its full name is Regulation (EU) 2019/1009.
- CE marking
- A mark on the packaging. It shows that the manufacturer states that the product follows the EU rules.
- EU fertilising product
- A fertilising product that carries the CE marking when it is sold. It can be sold in all EU countries.
- Technical documentation
- The set of documents that show the product follows the rules. It includes the product description, the materials, the production process, test reports and the label.
- Notified body
- An independent organisation that an EU country has approved to check products or quality systems. Some modules need one.
- Module
- One of the fixed routes for conformity assessment. The regulation has Module A, Module B followed by Module C, Module D1 and Module A1.
- Manufacturer
- The company that makes the product, or has it made, and sells it under its own name or brand.
Related articles
- What does a notified body need to assess my biostimulant trials?
- How many trials do I need for a plant biostimulant claim?
- Which claim fits my plant biostimulant?
- How do the trial setups differ per claim?
- How do I show the effect is not from nutrients?
- What are CMC and PFC?
- What are Module B and Module C?
- What goes into the technical documentation?
- What must be on the label?
